Detection of plasmid profile from MDR Pseudomonas aeruginosa isolate from wound infection

Abstract

Background: Antibiotic resistance is a major problem in treating infections in hospitals. Wound infections are one of the most common infections in hospitals and Pseudomonas aeruginosa is a predominant isolate which is usually multi drug resistant. Objective: To find out the antibiotic resistance pattern and plasmid profiling of resistant Pseudomonas aeruginosa isolated from wound infection. Methods: A descriptive type of cross-sectional study was carried out in the Department of Microbiology of Rajshahi Medical College and the Department of Botany of Rajshahi University, Rajshahi during the period from July 2014 to June 2015. A total of 150 wound swabs were collected from patients admitted in surgery department and its allied branches and cultured on appropriate bacteriological culture media.  Results: Culture had yielded growth in 131(87.33%) cases and Pseudomonas aeruginosa was 27(18%), Staphylococcus aureus was 22(14.66%). Escherichia coli was 56(37.33%), Proteus spp. was 19(12.67%), Klebsiella spp. was 7(4.67%) respectively. Antibiogram test was done on Pseudomonas aeruginosa with 7 different groups of antibiotics and found 4(14.81%) were resistant to 3 groups of antibiotics, 2(7.41%) were on 4 groups, 5(18.52%) were on 5 groups, 10(37.04%) were on 6 groups and 6(22.22%) were on 7 groups. A total of 27(18%) isolates were resistant to 3-7 groups of antibiotics. These isolates were further tested for plasmid detection and plasmid was responsible in 19(70.37%) resistant cases. Conclusion: All wound infections should be treated after performing antibiogram with adequate doses and duration.