Efficacy of a Vaccine Against Multidrug Resistant Escherichia coli in Mice Model

Abstract

Background: The emergence of multidrug resistant (MDR) Escherichia coli are particularly challenging for clinician. Development of an effective vaccine may overcome the problems associated with treatment failure and antibiotic resistance. Objective: To determine immune response against MDR E. coli using formaldehyde inactivated E. coli in mice model. Methods: In this study, fifteen Swiss albino mice were used. Intradermal inoculation was implemented three times at 14 days interval with formaldehyde inactivated MDR E. coli. The mice were given intraperitoneal challenge with live E. coli and observed for 14 days. Tail blood was collected to assess antigen binding capacity of the serum antibody of immunized mice by ELISA. Results: The survival rate was 100% among the mice of immunized group at 14 days of post challenge. In ELISA, OD values of serum immunoglobulin G of pre and post challenge immunized mice were significantly higher (p < .001). Conclusion: In this study, it was observed that formaldehyde inactivated MDR E. coli induced protective antibodies in mice model.