Genetic Detection of Carbapenemase Producing ImipenemResistant E. coli of Urine Sample in Dhaka Medical College

Abstract

Background: Antimicrobial resistance is a serious public health problem. The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. Objectives: This study aims to determine genetic detection of carbapenemase producing imipenem resistant E.coli in Dhaka Medical College & Hospital. Methods: A cross-sectional observational study was done over a period of one year in Dhaka Medical College and Hospital. The clinically suspected urinary tract infected ( UTI) patients attending at the outpatient department of Dhaka Medical College Hospital and the Microbiology Department of Dhaka Medical College constituted the study population. A total 280 urine samples were collected by purposive sampling with aseptic precaution from the attended patients. Antimicrobial resistant  patterns of uropathogenic E.coli with genetic detection of Carbapene- mase Producing Imipenem Resistant E. coli was determined by PCR in Microbiology Department Dhaka Medical College. Antibiotic suscepti-bility pattern was determined by double disk method for all the isolated E.coli strains. Results: A total of 280 urine samples, 83 (29.6%) samples were identified as culture positive. Among the microorganisms identified in this study, 77 (92.8%) were gram negative and the rest 6 (7.2%) were gram positive. The most resistance was found against Cotrimoxazole (90%) and lowest resistant was found against Tigecyline (6.67%). Genotyping detection of carbapenemase encoding genes among imipenem resistant uropathogenic Esch.coli, out of nine imipenem resistant Esch.coli, seven has positive encoding genes with 55.56% blaNDM-1 and 44.44% bla NDM-2 i.e., 22.22% bla VIM and 22.22% blaOXA-48. There was no bla IMP gene. Conclusion: Antimicrobial resistance has become a global issue now a days. So, we should use appropriate antibiotic according to the sensitivity pattern for bacteria to prevent emergence of resistance.