Current Microbial Isolates from Wound Swab and Their Susceptibility Pattern in Rajshahi Medical College Hospital

Abstract

Background: Wound infection is one of the major health issues that are caused and aggravated by harmful microorganisms where empiric treatment is routine. Objective: Current microbial isolates from wound swab and their susceptibility pattern in Rajshahi Medical College Hospital. Materials and Method: A total of 409 wound swab and pus samples were collected during the period from July 2024 to December 2024 at Rajshahi Medical College Hospital, Rajshahi, Bangladesh. Swabs from the wound were inoculated on appropriate media and cultured and the isolates were identified by standard procedures as needed. Antimicrobial susceptibility testing was performed by disk diffusion method according to ‘The Clinical and Laboratory Standard Institute’ guidelines. Results: In this study 266 bacterial isolates were recovered from 409 samples showing an isolation rate of 65%. The predominant bacteria isolated from infected wounds were Staphylococcus aureus 96 (36%) followed by Pseudomonas aeruginosa 64(24%), Escherichia coli 58 (22%), Klebsiella 28 (11%), CoNS 11(4%) and Proteus 9 (3%). Staphylococcus aureus was sensitive to Vancomycin (100%), Doxycycline (99.5%), Linezolid (97.1%). Among the Gram-negative Pseudomonas aeruginosa was predominant and showed sensitivity to imipenem (100%), piperacillin and tazobactam (79.6%), amikacin (70.39 %) and E coli showed sensitivity to imipenem (94.52%), levofloxacin (93.58%) Amikacin (88.1%). Conclusion: Staphylococcus aureus was the most frequently isolated pathogen from wound swab and the antibiotic sensitivity pattern of various isolates help to assist the clinician in appropriate selection of empirical antibiotics against wound infection.