Pattern and types of resistance in Carbapenemase Producing Imipenem Resistant E. coli of urine sample in Dhaka Medical College

Abstract

Background: Antimicrobial resistance is a serious public health problem. The emergence of metallo-β lactamase-producing Enterobacteriaceae is a worldwide health concern. Objectives: To determine pattern and types of resistance in Carbapenemase Producing Imipenem Resistant E. coli of urine sample in Dhaka Medical College. Methods: An observational study was carried out over a period of one year in Dhaka Medical College. A total 280 urine samples were collected purposive sampling with aseptic precaution from the attended suspected UTI patients. The collected specimens were inoculated in blood agar, nutrient agar and MacConkey agar media and incubated aerobically at 370C for 24 hours. Antibiotic susceptibility pattern was determined by double disk method for all the isolated E. coli strains. Results: A total of 280 urine samples, 83 (29.6%) samples were identified as culture positive. Among 83 culture positive urine, Esch.coli was identified as the most common (60, 72.29%) pathogens. Among the isolated uropathogenic E. coli, the most resistance was found against cotrimoxazole (90%) and lowest resistant was found against tigecyline (6.67%). Out of 60 uropathogenic Esch.coli 43 (71.67%) were multidrug resistance (MDR) followed by 14 (23.33%) and 3 (5.00%) were XDR and PDR respectively. Conclusion: Antimicrobial resistance has become a global issue now a days. So, we should use appropriate antibiotic according to the sensitivity pattern for bacteria to prevent emergence of resistance.