MDR Pseudomonas aeruginosa isolate from wound infection in Rajshahi Medical College Hospital

Abstract

Background: Emergence of resistant bacteria causing nosocomial infections increases the morbidity and mortality. Antibiotic resistance is a major problem in treating infections in hospitals. During the past decade, infecting bacteria that are resistant to several available antibiotics have emerged. The worldwide spread of multi drug resistant bacteria specially Pseudomonas aeruginosa is a predominant isolate which is usually multi drug resistant. Objective: To identify Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from wound infection. Methods: A descriptive type of cross-sectional study on wound infection was carried out in the Department of Microbiology of Rajshahi Medical College, Rajshahi during the period from July 2014 to June 2015. A total of 150 wound swabs were collected from patients admitted in surgery and its allied branches and cultured on appropriate bacteriological culture media. Results: Culture had yielded growth in 131(87.33%) cases and Pseudomonas aeruginosa was 27(18%), Staphylococcus aureus was 22(14.66%), Escherichia coli was 56(37.33%), Proteus spp was 19(12.67%), Klebsiella spp. was 7(4.67%) respectively. Antibiogram was tested on Pseudomonas aeruginosa with 7 different groups of antibiotics and found 4(14.81%) were resistant to 3 groups of drugs, 2(7.41%) were resistant to 4 groups, 5(18.52%) were resistant to 5 groups, 10(37.04%) were resistant to 6 groups and 6(22.22%) were resistant to 7 groups. A total of 27(18%) isolates were resistant to 3-7 groups of antibiotics. Conclusion: Pseudomonas aeruginosa is still a predominant isolate next to E. coli for wound infections. 27(18%) isolates were resistant to 3-7 groups of commonly used antibiotics. Recommendation: All wound infections should be treated only after performing antibiogram with adequate dose and duration of antibiotics.