Detection of metallo-beta-lactamase producing Enterobacteriaceae from wound infection in Rajshahi Medical College Hospital

Abstract

Background: Wound infection is a common problem and a wide range of bacteria including Enterobacteriaceae are responsible for it. Multidrug resistant Enterobacteriaceae are greatest risk for the management of wound infection as they produce beta-lactamase enzymes which cleaves beta-lactam drugs. Metallo-beta lactamase (MBL) is a member of beta-lactamase enzymes which are produced by gene mutation due to misuse of antibiotics. Objective: Detection of Metallo-beta-lactamase producing Enterobacteriaceae from wound infection. Methods: A descriptive type of study was carried out for the detection of MBL in the Departments of Microbiology and Surgery & its allied branches of Rajshahi Medical College and Hospital. A total 233 Enterobacteriaceae were isolated and antibiogram were done from wound swabs. The Enterobacteriaceae which showed resistant to both meropenem and ceftriaxone were used for the detection of MBL by double disk synergy test. Results: Among the Enterobacteriaceae, E. coli 86(70.49%), Proteus spp. 28(51.85%), Enterobacter spp. 27(72.97%), Klebsiella 8 (57.14%) and Providential spp. 3(50%), were resistant to both meropenem and ceftriaxone. Detected MBL. were 66(76.74%), 19(67.85%), 21(77.77%), 7(87.50%) and 1(33.33%) from E. coli, Proteus spp., Enterobacter spp., Klebsiella spp. and Provendentia spp. respectively. Conclusion: Multidrug resistant Enterobacteriaceae was due to production of MBL as result of mutation of genes for misuse of antibiotics without during antibiogram.